J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468

Research Paper

Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones

Takashi Kitano✉*, Choong-Gon Kim#, Naruya Saitou

Division of Population Genetics, National Institute of Genetics, Japan.
*Present address: Graduate School of Science and Engineering, Ibaraki University, Japan.
#Present address: Marine Ecosystem Research Center, Korea Institute of Ocean Science and Technology, Korea.

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Kitano T, Kim CG, Saitou N. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468. Available from http://www.jgenomics.com/v08p0080.htm

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We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.

Keywords: Fosmid library, Gorilla, HoxA