Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones

Kitano, Takashi; Kim, Choong-Gon; Saitou, Naruya

doi:10.7150/jgen.50468

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J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468 This volume Cite

Research Paper

Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones

Takashi Kitano^✉*, Choong-Gon Kim^#, Naruya Saitou

Division of Population Genetics, National Institute of Genetics, Japan.
*Present address: Graduate School of Science and Engineering, Ibaraki University, Japan.
#Present address: Marine Ecosystem Research Center, Korea Institute of Ocean Science and Technology, Korea.

Citation:

Kitano T, Kim CG, Saitou N. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468. https://www.jgenomics.com/v08p0080.htm

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Abstract

We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.

Keywords: Fosmid library, Gorilla, HoxA

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APA

Kitano, T., Kim, C.G., Saitou, N. (2020). Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. Journal of Genomics, 8, 80-83. https://doi.org/10.7150/jgen.50468.

ACS

Kitano, T.; Kim, C.G.; Saitou, N. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J. Genomics 2020, 8, 80-83. DOI: 10.7150/jgen.50468.

NLM

Kitano T, Kim CG, Saitou N. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468. https://www.jgenomics.com/v08p0080.htm

CSE

Kitano T, Kim CG, Saitou N. 2020. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J Genomics. 8:80-83.

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