Draft genome sequence of multi-resistant Salmonella enterica subsp. enterica serovar Rissen strain 19CS0416 isolated from Vietnam reveals mcr-1 plasmid mediated resistance to colistin already in 2013

Belen Gonzalez-Santamarina^1✉, Anne Busch^1,3, Silvia Garcia-Soto¹, Mostafa Y. Abdel-Glil¹, Jörg Linde¹, Reinhard Fries², Diana Meemken², Helmut Hotzel¹, Herbert Tomaso¹

1. Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses (IBIZ), Naumburger Str. 96a, 07743 Jena, Germany.
2. Institute of Food Safety and Food Hygiene, Section Meat Hygiene, Freie Universität Berlin, Königsweg 67, 14163 Berlin, Germany.
3. Department of Anesthesiology and Intensive Care Medicine, University Hospital Jena, Am Klinikum 1, 07747 Jena, Germany.

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Citation:
Gonzalez-Santamarina B, Busch A, Garcia-Soto S, Abdel-Glil MY, Linde J, Fries R, Meemken D, Hotzel H, Tomaso H. Draft genome sequence of multi-resistant Salmonella enterica subsp. enterica serovar Rissen strain 19CS0416 isolated from Vietnam reveals mcr-1 plasmid mediated resistance to colistin already in 2013. J Genomics 2020; 8:76-79. doi:10.7150/jgen.42790. Available from http://www.jgenomics.com/v08p0076.htm

Abstract

We report the first draft genome sequence of a Salmonella strain with plasmid-mediated resistance to colistin encoded by mcr-1 gene in Vietnam. Salmonella enterica subsp. enterica serovar Rissen was isolated from a Vietnamese pig slaughterhouse in 2013. We can confirm that mcr-1 gene is identical to the first reported mcr-1 gene of the Escherichia coli strain SHP45, isolated in 2015 from a Chinese pig. The plasmid containing this gene in the strain 19CS0416 was highly related (96.86% identity) to the plasmid (pHNSHP45) contained in this Chinese strain. Moreover, this plasmid was determined to be 100% identical to a plasmid (p13P477T-7) belonging to an Escherichia coli (13P477T) found in Hong Kong during the same year in pigs. Our results will aid in understanding the dissemination of mcr-1 gene in East Asia, dating back to as early as 2013.

Keywords: Salmonella Rissen, multidrug resistance, colistin, genome sequence, pig.