J Genomics 2019; 7:31-45. doi:10.7150/jgen.32164 This volume Cite


Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass

Ayyappa Kumar Sista Kameshwar1,2, Luiz Pereira Ramos2 Corresponding address, Wensheng Qin1 Corresponding address

1. Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario, P7B 5E1, Canada.
2. Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Universidade Federal do Paraná, P. O. Box 19032, Curitiba, Paraná, 81531-980, Brazil.

Kameshwar AKS, Ramos LP, Qin W. Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass. J Genomics 2019; 7:31-45. doi:10.7150/jgen.32164. https://www.jgenomics.com/v07p0031.htm
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Graphic abstract

Rumen is one of the most complex gastro-intestinal system in ruminating animals. With bountiful of microorganisms supporting in breakdown and consumption of minerals and nutrients from the complex plant biomass. It is predicted that a table spoon of ruminal fluid can reside up to 150 billion microorganisms including various species of bacteria, fungi and protozoa. Several studies in the past have extensively explained about the structural and functional physiology of the rumen. Studies based on rumen and its microbiota has increased significantly in the last decade to understand and reveal applications of the rumen microbiota in food processing, pharmaceutical, biofuel and biorefining industries. Recent high-throughput meta-genomic and proteomic studies have revealed humongous information on rumen microbial diversity. In this study, we have extensively reviewed and reported present-day's progress in understanding the rumen microbial diversity. As of today, NCBI resides about 821,870 records based on rumen with approximately 889 genome sequencing studies. We have retrieved all the rumen-based records from NCBI and extensively catalogued the rumen microbial diversity and the corresponding genomic and proteomic studies respectively. Also, we have provided a brief inventory of metadata analysis software packages and reviewed the metadata analysis approaches for understanding the functional involvement of these microorganisms. Knowing and understanding the present progress on rumen microbiota and performing metadata analysis studies will significantly benefit the researchers in identifying the molecular mechanisms involved in plant biomass degradation. These studies are also necessary for developing highly efficient microorganisms and enzyme mixtures for enhancing the benefits of cattle-feedstock and biofuel industries.

Keywords: Ruminating animals, Ruminal fluid, Microbiota, Genomic, Proteomic, Bacteria, Fungi, Protozoa.

1.0 Introduction

Ruminating animals feed on plant biomass for their complete nutrition. However, they do not secrete any cellulolytic or hemicellulolytic enzymes to breakdown the plant cell wall polysaccharides [1, 2]. Thus, ruminating animals are solely dependent on the rumen microbiota to enzymatically breakdown and ferment plant biomass for food and energy. Ruminating animals have a four-chambered stomach containing the following sections: (a) reticulum, (b) rumen, (c) omasum and (d) abomasum [1-3]. The reticulum and rumen are together considered as stomach of the ruminating animals and most part of the digestion occurs in the stomach [1-3]. The reticulum also called as blind pouch is the largest and first compartment of the ruminating animals' gut. The reticulum can hold up to 2.5 gallons of digested or undigested feed. It acts as a sieve when ruminating animals consumes indigestible materials like metal, plastic, etc. The honeycomb structure of the stomach wall stops their movement further into the digestive tract. The most significant feature of ruminating animals is their ability to regurgitate the feed, and this function happens to the feed which enters the reticulum [1-4].

The rumen is a hollow muscular organ of the gut, which grows large anatomically with the changing diet of a calf from milk to grass. Also, the microbial diversity of the rumen grows with the changing diets of a developing calf. In a fully grown ruminating animal, the rumen occupies the complete left section of the gut chamber [5, 6]. It is solely a fermentation chamber and it can contain up to 40-60 gallons of undigested food. Studies have proposed that a table spoon of ruminal fluid can contain 150 billion microorganisms including various species of bacteria, fungi, protozoa [3, 6, 7]. The rumen is well-suited for maintaining the growth of bacteria because it is an oxygen free environment with a pH between 5.8 to 6.4 and a temperature ranging between 100oF (37oC) to 108oF (42oC). A normal diet including portion of grains and forages should exhibit the above-mentioned pH range, which supports the growth of several bacterial strains in the rumen microbiota [5, 6, 8].

 Figure 1 

Pictorial representation of rumen and the process of digestion and absorption of the food material in ruminating animals [Note: The boxes are colored to represent the food passage from mouth to the rumen].

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The omasum, also called many plies due to its multi-layered muscular tissue, holds up to 4 gallons of the digesta [9]. This chamber removes the excess water from the digesta and reduces its particles size before it enters the abomasum [10-12]. The abomasum is the fourth and final true glandular compartment of the gut and it is considered as the true stomach of the ruminating animals' gut as it secretes several digesting enzymes [5, 6, 13, 14]. It can contain up to 5 gallons of feed material, but the digesta remains for a lesser period in the abomasum when compared to that of rumen. The presence of food in the abomasum stimulates the production of hydrochloric acid and this converts pepsinogen to pepsin, which converts proteins to shorter peptides and aminoacids for the digestion and absorption into the small intestine [13, 15]. The pH of the stomach is maintained between 2 to 4 because of the secretion of strong acids. The digested food and released nutrients pass from the abomasum to the small intestine and, as a result, the pH rises at a slow rate. This physiological rise in pH has its implications on the pancreas and intestinal mucosa as the enzymes released by it are only active at neutral or slight alkaline pH (Figure 1) [1, 5, 6, 11, 16].

The bile salts produced in the liver helps maintaining the alkaline pH of the small intestine [17, 18]. These bile salts separate the fat globules and provide the lipase enzymes more surface area to act on. The bile and pancreatic secretions maintain the neutral pH of the gastric juice, providing the optimum conditions for enzymes to hydrolyze starch, proteins and lipids. The small intestine is the major absorption site for the nutrients obtained from the metabolism [19]. When ruminants are fed with high forage diets, most of the starch and soluble sugars are fermented by the microbial community of the rumen. However, when ruminants are fed with higher amounts of grain diets, about 50 percent of the dietary starch escape from the rumen to the lower gastrointestinal tract where it is digested. Thus, substantial amounts of glucose and other monosaccharides are absorbed by the small intestine [19, 20]. The proteins absorbed by the small intestine are derived from three sources, which can be classified as (a) dietary proteins (escaped from the microbial fermentation in the rumen), (b) microbial proteins present in the cells and (c) endogenous proteins (sloughed cells and secretions of abomasum and intestine). These proteins are further digested to small peptides and aminoacids by the pancreatic and intestinal proteases, which are absorbed by the small intestine as well [21]. The lipids reaching the small intestine are majorly esterified fatty acids and phospholipids. Pancreatic lipases readily hydrolyze the esterified fatty acids produced by microbes and triglycerides escaped from the ruminal microorganisms. Thus, the released free fatty acids are easily absorbed by the mucosal cells of the small intestine [5, 16, 17, 19] (Figure 2).

 Figure 2 

Breakdown products obtained from the process of fermentation by rumen microbiota. [Note: The uncolored boxes are the macromolecules and the blue colored boxes represent the products of metabolism].

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The feed that escapes from the above-mentioned digestion process enters the large intestine, where water, minerals, nitrogen and volatile fatty acids are absorbed [16]. The major functions of the large intestine include (a) balancing electrolytes, (b) perform microbial fermentation and (c) provide a temporary storage of excreta. Any undigested feed obtained from the gastrointestinal tract will be passed out in the feces. Usually the fecal matter contains undigested feed, metabolic nitrogen, undigested fat and some microorganisms [5].

1.1 Rumination

Rumination significantly supports the process of digestion. The processes involved during rumination are (a) the regurgitation of food and (b) the rechewing, re-salivation and re-swallowing of the ingested food material [8, 10, 22]. The rumination process reduces the particle size of the ingested food, which significantly enhances microbial fermentation and aids in easy passage by the stomach compartments. Rechewing or ruminating strongly induces the process of salivation. A mature ruminant produces 47.5 gallons of saliva per day if it chews the feed for 6 to 8 hours [1, 23-25]. Thus, secretion of saliva is directly proportional to the amount of time a cow spends in chewing or ruminating the bolus or cud. The saliva of ruminating animals highly comprises of sodium (126 mEq/L), phosphate (26 mEq/L), bicarbonate (126 mEq/L) and lesser amounts of potassium (6 mEq/L) and chloride (7 mEq/L) ions which act as buffering agents in the digestive system [1, 24, 26]. It aids in the maintenance of the neutral environment by neutralizing the acids released during fermentation. The type of feed ingested also plays a crucial role in the stimulation of salivation. Dried feeds such as hay and grass stimulate higher rates of salivation and other feeds such as silage, fresh grass and pelleted materials result in lesser stimulation of saliva. It was also reported that rate of saliva production is significantly reduced if the ruminants are not fed with adequate amounts of effective fiber and high moisture containing feeds [8, 10, 22].

1.2 Functional Physiology of Rumen

The rumen is a muscular organ which performs the mixing and churning of the digesta [1]. The rumen constantly stirs the digesta to enhance the accessibility of coarser feed particles during the process of regurgitation and bolus chewing, thus reducing the particle size of feed and enhancing its fermentation [27]. Smaller feed particles digested by the rumen are collected at the bottom and eventually pass out of the rumen along with the microbial strains to aid digestion in the lower gastrointestinal tract [5, 28]. The composition of the ruminal contents is completely dependent on the type of food materials that are ingested. Generally, ruminants consume different types of food. Thus, contents of the rumen are not uniform among ruminants. Since the rumen is a fermentation chamber, gases such as hydrogen, oxygen, nitrogen, methane and carbon dioxide are produced during microbial fermentation [1]. The descending order of gases released in the rumen are carbon dioxide (65.5%), methane (26.8%), nitrogen (7.0%), oxygen (0.5%) and hydrogen (0.2%) [1, 29]. The released gases get accumulated in the upper part of the rumen with higher percentages of carbon dioxide and methane.

The composition of these gases is again dependent on the ecology and fermentation rates of the rumen; usually, the proportion of carbon dioxide is 2-3 times higher than that of methane [7, 28]. However, a large proportion of carbon dioxide is in turn reduced to methane. On average, about 132 to 264 gallons of these gases are produced by the process of fermentation, which are belched frequently by the ruminants to avoid bloating [5]. Rumen mucosal linings consists of ruminal papillae (organs for absorption) and their size and distribution are directly related to the type of food material they ingest. The diet of ruminating animals varies significantly from high forage diet to a high grain diet. Changes in diet must be implemented gradually to permit the adaptation of papillae to changes in nutrition, which may take approximately 2 to 3 weeks [30]. The ruminal papillae are also related to the production acids from the fermentation of feeds. Thus, the size, number and distribution of the ruminal papillae depends on the diet and acids released during fermentation [31].

1.3 Microbiology of Rumen

Ruminating animals are very rich in microorganisms. Studies have reported that a milliliter of rumen contains approximately 105 to 106 protozoans and about 1010 to 1011 of bacterial inhabitants [1-3, 7]. The protozoans inhabiting ruminal fluids can be majorly divided into two ciliate groups: (a) holotrichs and (b) entodinimorphs [32]. The bacterial population present in ruminal fluids are classified into cocci, rods and spirilla based on their size and shape [16, 25]. The ruminal bacteria can also be classified based on their substrate fermenting abilities into eight different groups that are able to consume cellulose, hemicelluloses, starch, simple sugars, pectin, proteins, lipids and intermediate acids. The ruminal bacteria are also majorly known for their ability to produce methane [1, 32, 33]. Most of the ruminal bacteria are capable of degrading and fermenting multiple substrates. Compared to other ruminal microorganisms, methane producing bacteria are the most important and special bacteria that regulate the process of fermentation and its products in the rumen [34]. Methanogens aid in removing hydrogen gas by forming methane and reducing carbon dioxide, thus maintaining the lower concentrations of hydrogen in the rumen and supporting the growth and development of several other bacterial strains [33, 35].

Apart from bacteria, protozoa are highly observed in the ruminal fluid when the ruminants are fed with higher digestibility feed [33, 35]. Thus, different diets of the ruminants encourage the growth of different protozoan populations. Diets richer in starch and soluble sugars promote higher growth rates of different protozoan populations. Protozoans are important in neutralizing the rumen environment by stabilizing the end products of fermentation [36]. Recent studies have reported on the occurrence of anaerobic fungi in the ruminal fluids of ruminants. Especially, the fungal divisions Chytridiomycota and Neocallimastigomycota were found to contain anaerobic fungi which are also majorly observed in the rumens of the ruminating animals [36, 37]. Studies have reported that anaerobic fungi contribute up to 8% of the microbial mass in the rumen. The anaerobic fungi were proposed to play a significant role in degradation of plant biomass containing cellulose and xylans. However, further studies are still needed to demonstrate the role of anaerobic fungi in the rumen metabolism [36, 38-40].

Physiologically, the rumen comprises three connecting environments, one liquid (free living microorganisms in the rumen liquid breakdown and feed on carbohydrates and protein) and another solid (microorganisms attached to the ingested food particles and to the rumen epithelium or protozoa) [1, 5]. Liquid and solid phase microorganisms constitute up to 70% of the rumen microbiota and up to 5% of microorganisms associated with the rumen epithelium [41]. To maintain their population, it is important for the bacteria to have a reproduction time shorter than the turnover of the rumen digesta. However, bacteria with slower reproduction rates tend to attach to the particulate matter and start degrading it, without being flushed out of the body in the liquid stream [1, 33]. Majorly, two factors strongly influence the bacterial population in the rumen: (a) the type of food material they ingest and (b) the rumen pH [3, 22]. It is highly important to consider the reproduction rates of microorganisms when shifting the diet of ruminants, as changing diet also requires a change in shift of microbial population in the rumen. Microbial shift of the rumen is a time taking process and it requires several days. For instance, when ruminants are fed with simple carbohydrates (easily fermentable), such feeding habit encourages the growth of bacteria with lactate utilizing and producing abilities; hence, acid-sensitive lactate utilizing bacteria are gradually replaced by acid-tolerant lactate utilizing bacteria [25, 29, 33, 42].

It is highly necessary to maintain ideal pH conditions for sustaining the microbiota of the rumen. Majorly, bacteria can be classified on the basis of their activity in the rumen as fiber digestors (active at pH 6.2 to 6.8) and starch digestors (active at pH 5.2 to 6.0) [16]. Studies have also reported that the population of cellulolytic and methanogenic bacteria drops if the rumen pH drops below 6.0 and few species of protozoa were also found to drop if the rumen pH is under 5.5. Several studies have reported on the major bacterial strains involved in the metabolism of plant biomass components in ruminating animals (Figure 3).

2.0 Metadata Analysis (or) Systematic Review Based Methods

The metadata analysis and systematic review are two statistical approaches for re-analyzing the data by combining the information from various datasets and identifying the common effect behind the effect size or treatment effect respectively. Metadata analysis (or) systematic review is mainly used for understanding and identifying the reason responsible for the variations in the effect size between one study to another study respectively. The valuable information reported in previous literature, and the resourceful supplementary information are major sources for the systematic reviews and metadata analysis approaches. There are various advantages of metadata analysis approaches such as: a) It plays a significantly role in designing and executing new studies, b) metadata analysis helps in identifying the questions which are already reported, and it can help the studies to focus on the answered questions of the research. In our present study, we have conducted an extensive systematic review reporting all the previously reported literature related to the “rumen” and “rumen microbiota”, which can be further used by the data scientists and researchers to identify the microorganisms supporting the metabolism of lignocellulosic plant biomass in cattle.

 Figure 3 

The major microorganism species reported in the previous studies and classified based on the substrates they degrade.

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 Figure 4 

Pictorial representation of the present days collection a) the number of records and datasets in NCBI database based on the term “Rumen”, b) briefly lists details of the genomic and metagenomic sequencing studies i) sequencing institution, ii) level of assembly and iii) the classification of assembled genomes into archaea and bacteria groups and c) shows the division of assembled genomes of microorganisms at genus level (A, B, D, E and G - proteobacteria represents α, β, δ, γ, £- proteobacteria). [Note: The color gradient used for the images A and B were generated using Microsoft Excel with red color represents lowest count, green color represents highest count and yellow color represents for intermediate count. Especially for figure 4A was gradient colored based for each section separately].

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2.1 Literature Related to Rumen

Understanding the structural and functional physiology of the rumen and its diverse microbiota are subject of study since several years. Understanding the characteristic features of rumen has various potential benefits such as (a) increase the rate of breakdown of ingested feed, (b) increase the rate of absorption of nutrients and minerals by ruminants and (c) diversify the countless commercial applications of rumen microbiota in the food, pharmaceutical, textile, biofuel and bioremediation industries. The NCBI database is an online public repository for biological and life science data. The NCBI database resides and classifies the data among 37 NCBI databases (Table 1). The search with the term “Rumen” has resulted in the data distributed among the 27 databases respectively, with zero records from the NCBI Genetics database.

Several research communities around the world have contributed significantly to the current knowledge on the structural and functional physiology of the rumen. We have retrieved and reported the total number of 821,870 recorded datasets on “rumen” till date, classified among different NCBI databases. The 821,870 articles of NCBI can be classified under sections of literature-25,701, genome-4,97,446, genes-60,950, proteins-2,37,722 and chemicals-51 of the reported articles among these databases respectively. Till date in NCBI literature section there are 25,701 reported articles which includes 124 books, 15,449 Pubmed articles, 10,038 Pubmed central articles, 3 Pubmed health, 1 medical subject headings, 1 online mendelian inheritance in man and 35 national library of medicine articles respectively (Figure 4A).

 Table 1 

The NCBI public repository and its major classification of NCBI databases.

NCBI LiteratureBookshelf, MeSH, NLM Catalog, PubMed and PubMed Central
NCBI GenesGene, GEO Database, GEO Profiles, HomoloGene, PopSet UniGene
NCBI GeneticsClinVar, dbGap, dbSNP, dbVar, GTR, MedGen, OMIM
NCBI GenomesAssembly, BioCollections, BioProject, BioSample, Genome, Nucleotide, Probe, SRA, Taxonomy
NCBI ProteinsConserved Domains, Identical Protein Groups, Proteins, Protein Clusters, Sparcle, Structure
NCBI ChemicalsBiosystems, PubChem BioAssay, PubChem Compound, PubChem Substance

2.2 Metagenomic, Genomic, Proteomic Studies on Rumen and Its Microbiota

In the past, numerous studies were conducted to understand the microbial diversity of the rumen and its fermenting abilities by employing conventional isolation techniques and biochemical characterization methods [32, 43-46]. These microorganisms were characterized majorly by their fiber digestion and protein assimilation abilities [32]. Using these targeted screening approaches, several rumen microorganisms were reported in the past, including bacterial, fungal and protozoan species [44, 47]. Over time, the conventional standard phylogenetic analysis based on 16s RNA [47-50] and 18s RNA [50-52] functional gene classification methods were applied. However, these techniques are very limited, and they only provide the functional relatedness of the targeted species to the already defined and studied phenotypes. Development of advanced molecular techniques especially high throughput sequencing has replaced these conventional methods with the whole genome and metagenome sequencing methods [53-63]. Recent developments in the field of genome sequencing revealed important facts about living organisms and the molecular mechanisms underlying the cellular processes [64-66]. In the last few years, whole genome sequences of several commercially important bacterial and fungal species have been revealed. In 2013, the first whole genome sequence of rumen bacteria Wolinella succinogens was revealed and after that several other rumen bacterial and fungal genomes have been released [67].

More recently, several studies have been conducted to understand and reveal the complete genomic sequences of various rumen microorganisms. Simultaneously, public data repositories such as DOE-Joint Genome Institute (https://jgi.doe.gov/) [68, 69] and Hungate 1000 (aimed to sequence 1000 rumen microbial strains including rumen bacteria, methanogenic bacteria, fungi, archaea and ciliate protozoans - http://www.rmgnetwork.org/hungate1000.html) were made available [70]. The Hungate research project has 410 whole genome sequences of microorganisms present in the rumen of cattle. This project involves 60 laboratories from 14 research organizations situated across 9 countries. The collection of whole genome sequence information of these 410 rumen microorganisms can be retrieved from the DOE-Joint genome institute portal https://genome.jgi.doe.gov/portal/HungateCollection/HungateCollection.info.html [70].

Currently, the NCBI-Assembly harbors 889 whole genome sequences or clone-based assembly sequences of rumen microorganisms and rumen metagenome, respectively. We have retrieved all the summary data from the NCBI-Assembly and separated the data based on the organism, submitter and assembly level. Out of these, 859 are bacterial genomes and 29 are archaeal genomes. Based on the assembly level these sequenced genomes can be separated into 591 scaffold level and 298 contig level. Most number of genomes were submitted by the Roslin Institute (886 genomes), 2 genomes came from the University of Vienna and 1 genome from the DOE-Joint Genome Institute (Figure 4B). The 859 sequenced rumen bacterial genomes can be majorly separated into 439 firmicutes, 323 CFB (Cytophaga-Flavobacterium-Bacteroides) group bacteria, 17 high GC gram positive and 14 actinobacteria (Figure 4C). Among the 889 sequenced rumen microorganisms only 9 bacterial strains are cultured and 879 were uncultured strains. Interestingly, among the 879 uncultured strains, 434 were firmicutes (276 Clostridiales, 74 Lachnospiraceae, 28 Erysipelotrichaceae, 15 Selenomonadales, and 10 Ruminococcus) and 323 were CFB group bacteria (135 Prevotellaceae, 121 Bacteroidales and 67 Prevotella).

 Table 2 

List of the cultured and uncultured NCBI assembled genomes of the rumen isolated microorganisms retrieved from NCBI Assembly Database.

NCBI Assembled genomes of Microorganisms retrieved from Rumen
Acidaminococcus fermentans, Bacillus licheniformis, Kandleria vitulina, Megasphaera sp. DJF_B143, Streptococcus equinus
Uncultured Firmicutes: Acidaminococcus sp., Clostridia bacterium, Clostridiales bacterium, Dialister sp., Erysipelotrichaceae bacterium, Eubacterium sp., Firmicutes bacterium, Lachnospiraceae bacterium, Negativicutes bacterium, Ruminococcus sp., Selenomonadales bacterium, Sharpea sp., Streptococcus sp., Veillonellaceae bacterium
Uncultured actinobacteria: Olsenella species
Uncultured α-Proteobacteria: Rhodospirillaceae bacterium
Uncultured Bacteria: Brachyspira sp., Elusimicrobia bacterium, Elusimicrobium sp., Fibrobacter sp., Lentisphaerae bacterium
Uncultured β-Proteobacteria: Sutterella sp.
Uncultured CFB group bacteria: Bacteroidales bacterium, Prevotella species, Prevotellaceae bacterium
Uncultured γ-Proteobacteria: Desulfovibrio species
Uncultured ε-Proteobacteria: Campylobacter species
Methanomassiliicoccales archaeon M1 and M2 strain
Uncultured Euryarchaeotes: Candidatus Methanomethylophilus species, Methanobrevibacter species, Methanosphaera species
Uncultured δ-Proteobacteria: Acinetobacter species, gamma proteobacterium, Succinatimonas species,
Bifidobacterium merycicum, Uncultured high GC gram+: Actinobacterium species, Bifidobacterium species
Mycoplasmas: Mycoplasmataceae bacterium
Planctomycetes: Planctomycete species
Spirochetes: Spirochaetaceae bacterium, Treponema species

Recent next generation sequencing methods especially large-scale metagenome sequencing techniques has enabled the identification of microbial communities inhabiting the complex environments such as rumen (Figure 4C, Table 2). However, the metagenome sequencing techniques skips various traditional identification methods such as isolation, culturing and characterization of microorganisms. Thus, majority of the bacterial strains reported from the NCBI database are reported as uncultured strains respectively.

Earlier studies have reported that rumen bacteria predominantly include gram negative cellulolytic bacteria such as Fibrobacter succinogenes, Ruminococcus flavifaciens, Megasphaera elsdenii, Selenomonas ruminantium, Veillonella parvula, Butyrivibrio fibrisolvens Lactobacillus ruminis respectively. According to Jewell, K. A, et al (2015), rumen microbiota of cow's rumen majorly composed of Bacteroidetes- 49.42%, Firmicutes-39.32%, Proteobacteria-5.67%, and Tenericutes-2.17% respectively [71]. This study has also reported that the abundant genera of cow's rumen includes Prevotella (40.15%), Butyrivibrio (2.38%), Ruminococcus (2.35%), Coprococcus (2.29%), and Succiniclasticum (2.28%) genera respectively [71].

The NCBI search with the term “Rumen” has resulted in a total of 1,026 records were listed under the NCBI BioProjects section, out of which 641 records are mono-isolates, 53 records are multi-species, 42 are multi-isolates, 274 are environmental samples and 14 are other reports. Based on the type of project, these data can be further classified into 232 nucleotide, 49 protein, 222 assembly, 764 SRA (sequence read archive) and 29 gene expression omnibus datasets (GEO). Among the 641 mono-isolate cultures, the following genuses were highly observed: Butyrivibrio, Lachnospiraceae, Streptococcus, Prevotella, Clostridium, Ruminococcus, Pseudobutyrivibrio, Olsenella, Selenomonas, Bifidobacterium, Eubacterium, Ruminococcaceae, Bacteroides, Methanobrevibacter, Lactobacillus and Enterococcus (Figure 5). The systematic review of the NCBI Genomes databases reported in this study adds up to the present days knowledge on rumen microbial communities.

The genome database of the NCBI harbors 21 genomes of the following bacteria: Treponema saccharophilum, Selenomonas ruminantium, Slackia heliotrinireducens, Butyrivibrio proteoclasticus, Wolinella succinogenes, Fibrobacter succinogenes, Lachnospiraceae bacterium, Desulfotomaculum ruminis, Ruminococcus champanellensis, Ruminococcus bromii, Butyrivibrio fibrisolvens, Oxalobacter formigenes, Holdemanella biformis, Lactobacillus ruminis, Eubacterium saphenum, Eubacterium rectale, Prevotella ruminicola, Sagittula stellata, Actinobacillus succinogenes, Eubacterium eligens and Ruminococcus albus (Table 3). The genome survey sequences (GSS) are similar to the expression sequence tags (EST) but GSS nucleotide sequences are of genomic origin, whereas EST nucleotide sequences are of mRNA origin. As of today, there are 204 rumen nucleotide sequences of GSS origin, out of which 81 (GSS: HHX01H12) are sequences from rumen metagenome from uncultured organisms, 50 sequences belong to Orpinomyces sp. OUS1, 64 sequences belong to bovine rumen metagenome, 3 sequences belong to Gastrothylax crumenifer and 6 sequences belong to Paramphistomum cervi.

 Figure 5 

The project data details of rumen microorganisms and the highly occurring genus in the rumen isolates. [Note: The color gradient used for the images A and B were generated using Microsoft Excel with red color represents lowest count, green color represents highest count and yellow color represents for intermediate count].

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 Table 3 

Lists the details of ruminal bacteria with whole genome sequences, available from the genome database of NCBI.

NameSubgroupLength (Mb)ProteinGC%
Treponema saccharophilumSpirochaetia3.4539283753.2%
Selenomonas ruminantiumFirmicutes3.1123280550.1%
Slackia heliotrinireducensActinobacteria3.1650272160.2%
Butyrivibrio proteoclasticusFirmicutes4.0483350440.149%
Wolinella succinogenesδ/ε subdivision2.1103204048.5%
Fibrobacter succinogenesFibrobacteres3.8428307748.05%
Lachnospiraceae bacteriumFirmicutes2.9589244654.3%
Desulfotomaculum ruminisFirmicutes3.9690375347.2%
Ruminococcus champanellensisFirmicutes2.5424205953.35%
Ruminococcus bromiiFirmicutes2.2760212141%
Butyrivibrio fibrisolvensFirmicutes4.6654376439.7%
Oxalobacter formigenesβ-proteobacteria2.4528220449.06%
Holdemanella biformisFirmicutes2.5177226134.4%
Lactobacillus ruminisFirmicutes2.0249184643.4%
Eubacterium saphenumFirmicutes1.084991940.6%
Eubacterium rectaleFirmicutes3.3450297341.5%
Prevotella ruminicolaBacteroidetes3.5894290847.7%
Sagittula stellataα-proteobacteria5.2628481665%
Actinobacillus succinogenesγ-proteobacteria2.3170210444.9%
Eubacterium eligensFirmicutes2.8313261337.56%
Ruminococcus albusFirmicutes3.8452366144.5%

The nucleotide database harbors a total of 468616 nucleotide sequences which can be majorly classified based on their source as gut metagenome (201684), uncultured bacterium (169318), uncultured rumen bacterium (18860), uncultured archaeon (16876), Clostridium beijerinckii (6121), uncultured prokaryote (6090), Ruminococcus flavefaciens (2582), uncultured rumen protozoa (1975), Acinetobacter baumannii (1929), uncultured organism (1418), uncultured methanogenic archaeon (1316), uncultured Prevotella sp. (1207), Pseudomonas aeruginosa (1180), uncultured rumen archaeon (1175), uncultured fungus (1067), Pseudochrobactrum sp. AO18b (1015), uncultured Methanobacteriaceae archaeon (962), Bacillus nealsonii (817), Lactococcus lactis (811), Synergistes jonesii (709) and all other taxa (31504), respectively. The sequence read archive (SRA) database currently contains 16,645 records which can be majorly classified into bovine gut metagenome (6558), gut metagenome (3901), stomach metagenome (1300), Bos taurus (1267), bovine metagenome (754), Ovis aries (331), metagenome (249), sheep gut metagenome (202), environmental samples (182), Bubalus bubalis (151), anaerobic digester metagenome (134), metagenomes (13419), uncultured prokaryote (90), feces metagenome (70), rumen bacterium 1/9293-11A (66), Bos indicus (58), firmicutes (405), fermentation metagenome (51), Capra hircus (43), synthetic metagenome (38) and all other taxa (1070), respectively.

The expressed sequence tags (EST) database holds a collection of short single-read transcripts from GenBank. These transcript sequences deliver the means for determining the gene expression, for finding the possible genetic variations and for annotating gene products. As of today there are 36,535 EST records in the NCBI EST database which can be classified as Bos taurus (32027), Entodinium caudatum (1061), Polyplastron multivesiculatum (624), Epidinium ecaudatum (594), Dasytricha ruminantium (587), Isotricha prostoma (542), Eudiplodinium maggii (542), Isotricha sp. BBF-2003 (276), Metadinium medium (151), Isotricha intestinalis (82), Entodinium simplex (27), Diploplastron affine (10), uncultured microorganism (6), Homo sapiens (4), Oryzias latipes (1) and Leucoraja erinacea (1), respectively.

The NCBI Gene database harbors and integrates a wide range of information about the gene sequences of a wide range of species. Currently, there are 17,937 records about the gene sequences of various rumen microorganisms, which can majorly be classified into Methanosarcina barkeri CM1 (3764), Methanobacterium formicicum (2420), Methanobrevibacter millerae (2318), Methanobrevibacter sp. YE315 (2146), Methanogenic archaeon ISO4-H5 (1868), Methanobrevibacter olleyae (1868), Methanobrevibacter sp. AbM4 (1737), Thermoplasmatales archaeon BRNA1 (1526), Calicophoron microbothrioides (36), Bos taurus (15), Streptomyces atroolivaceus (6), Escherichia coli (4), Streptomyces scabiei 87.22 (4), Streptomyces rimosus subsp. rimosus (4), Streptomyces canus (4), Streptomyces leeuwenhoekii (4), Streptomyces olivochromogenes (4), Ovis aries (3), Colletotrichum orchidophilum (3), Sphingobium yanoikuyae ATCC 51230 (3) and all other taxa (200), respectively.

The gene expression omnibus (GEO) is a public repository of functional genomics data. The GEO repository contains both microarray and gene sequence (RNA-Sequencing) datasets. Present day GEO database contains 34 gene expression datasets and a total of 529 gene expression data samples and 64 gene expression profiles (Table 4). The term “rumen” is enriched with 15 conserved domain sites upon our search in the NCBI database. There is a total of 3,814 records in NCBI identical protein database groups, whose annotated protein sequences are available in GenBank, RefSeq, SwissProt and Protein data bank. This allows researchers to rapidly obtain information about the protein of interest. A total of 233,827 protein sequences are publicly available in the NCBI-Protein database, which can be majorly classified as bacterial origin (203479), RefSeq (4414) and related structures (22525). NCBI-SPARCLE is a database which functionally characterizes and labels the protein sequences based on their unique conserved domains. NCBI-SPARCLE presently contains 29 records related to the rumen. There are 45 protein structures related to the rumen and ruminal microorganisms till date in the NCBI-protein database (Table 5).

2.3 NCBI Chemicals Database

The NCBI Chemical database is a public repository for harboring the chemical information and molecular pathways. It provides a direct links to the relevant records such as proteins, genes and other participating compounds in other NCBI databases. The NCBI chemical database can be majorly classified into three domains: (a) chemical assays, (b) biological assays and (c) molecular pathways. We have searched for the publicly available resources that are related to the rumen. A total of 51 resources were available out of which 13 were listed under BioSystems (it provides information about molecular pathways and links to the proteins, genes and chemicals), 15 were listed under PubChem BioAssay (it includes bioactivity screening studies) and 23 were listed under PubChem Substance (it harbors information about chemical substances) (Table 6).

 Table 4 

Lists the details of gene expression datasets that was retrieved from the NCBI-GEO database using the term “rumen”.

GSE89874Bos taurusRNA-SequencingIllumina HiSeq 200045
GSE81847Ovis ariesRNA-SequencingIllumina HiSeq 200062
GSE107550Fibro-chipMicroarray (Rumen microbiota)Custom Agilent gene expression array14
GSE89162Bos taurusRNA-SequencingIllumina HiSeq 200038
GSE78197Bos taurusRNA-SequencingIllumina HiSeq 200018
GSE99066Capra hircusRNA-SequencingIllumina HiSeq 25009
GSE76346Bos taurusRNA-SequencingIllumina HiSeq 200010
GSE86323Bos taurusRNA-SequencingIllumina HiSeq 20008
GSE93907Fibrobacter succinogenesRNA-SequencingIllumina MiSeq10
GSE76501Bos taurusRNA-SequencingIllumina HiSeq 200020
GSE80173BacteriaMicroarray (CAZy-chip)Custom Agilent gene expression array94
GSE52193Bos taurusRNA-SequencingIllumina HiScanSQ63
GSE87391Bos taurusMicroarrayAffymetrix Bovine genome array10
GSE83813Bos taurusMicroarrayAgilent Bovine custom array7
GSE82272Bos taurusMicroarrayAgilent Bovine custom array8
GSE74329Bos taurusRNA-SequencingIllumina HiSeq 200071
GSE74379Bacteroides xylanosolvensRNA-SequencingIllumina HiSeq 200024
GSE68791Ovis ariesRNA-SequencingIllumina MiSeq2
GSE71153Bos taurusRNA-SequencingIllumina Genome Analyzer II16
GSE63550Bos taurusRNA-SequencingIllumina HiSeq 20004
GSE62624Bovine gut metagenomeMicroarrayCUST Rumen Bacto-array12
GSE50448Ovis ariesMicroarrayCustom Agilent gene expression array8
GSE39206BacteriaSARST librariesSARST libraries9
GSE35212Bos taurusMicroarrayAffymetrix Bovine genome array14
GSE21492Synthetic constructMicroarrayNimbleGen Custom array3
GSE16747Escherichia coliMicroarrayEDL933 Spotted PCR array7
GSE19802Bos taurusMicroarrayCustom Bovine array10
GSE21544Bos taurusRNA-SequencingIllumina Genome Analyzer95
GSE18716Methanobrevibacter ruminantiumMicroarrayCustom microarray1
GSE18382Bos taurusMicroarrayCustom Bovine microarray18
GSE17849Bos taurusMicroarrayAffymetrix Bovine Genome array12
GSE15916Ruminococcus flavefaciensMicroarrayCustom microarray4
GSE3029Bos taurusMicroarrayCustom microarray39
GSE1842BacteriaSARST librariesSARST libraries1

2.4 Application of Metadata Analysis Work-frame

The metadata analysis is statistical data analysis approach which involves systematic analysis of data generated from multiple studies [72, 73]. The metadata analysis approach can significantly reveal about the regular involvement of highly active genes or proteins involved in a molecular mechanism. Presence of genomic and proteomic data based on rumen allows data scientists and bioinformaticians to extensively analyze the metadata of various rumen microorganisms. The genome sequencing studies reveal enormous genetic information. Comparative analysis of annotated genome can primarily reveal significant information about the evolutionary loss of genes, broad involvement of these genes (or) proteins in various molecular mechanisms. Biological annotations such as especially InterPro, GO (Gene Ontology), KOG (Eukaryotic orthologous groups), COG (Prokaryotic orthologous groups), CAZy (Carbohydrate active enzymes), SM (Secondary metabolites), KEGG (Kyoto encyclopedia of genes and genomes) and MEROPS (peptide database) helps to compare the genomic data. Metadata analysis of anaerobic fungal genomes belonging to Neocallimastigomycota division (Anaeromyces robustus, Neocallimatix californiae, Orpinomyces sp, Piromyces finnis, Piromyces sp E2) has revealed about extensive loss of genes involved metabolism of ligninolytic genes [74]. It was also reported that these anaerobic fungi encode for arsenal of enzymes which are involved in breakdown and conversion of plant cell wall carbohydrates. This metadata analysis study also reported that these anaerobic fungi possess highest number of carbohydrate active enzymes compared to any other fungal species [74]. Similarly, metadata analysis study of different wood-decaying fungi (white-rot, brown-rot and soft-rot fungi) has reported about the genes and proteins underlying various molecular mechanisms employed during metabolism of plant biomass components [75]. This study has also extensively reported and compared the total cellulolytic, hemicellulolytic, pectinolytic and ligninolytic abilities of white, brown and soft rot fungi [75] (Figure 6).

 Table 5 

List of the details of characterized proteins with corresponding structural details and source organism, available from the NCBI genome database.

PDBMMDBStructural detailsOrganism
5K9H143569Glycoside Hydrolase 29Rumen bacterium
4DEV107545Acetyl Xylan EsteraseButyrivibrio proteoclasticus B316
3U37107485Acetyl Xylan EsteraseButyrivibrio proteoclasticus B316
5WH8160168CellulaseParaporphyromonas polyenzymogenes
5U22151772Glycoside Hydrolase 39Neocallimastix frontalis
5G0R149432Methyl-coenzyme-M-ReductaseMethanothermobacter marburgensis
2WTN79382Feruloyl esteraseButyrivibrio proteoclasticus
2WTM79381Feruloyl esteraseButyrivibrio proteoclasticus
5K6O143776Glycoside Hydrolase 3Rumen metagenome
5K6N143775Glycoside Hydrolase 3Rumen metagenome
5K6M143774Glycoside Hydrolase 3Rumen metagenome
5K6L143773Glycoside Hydrolase 3Rumen metagenome
4NOV123603Glycoside Hydrolase 43Butyrivibrio proteoclasticus B316
4KCB117247Glycoside Hydrolase 5Uncultured bacterium
4KCA117246Glycoside Hydrolase 5Bos taurus
5LXV144187Scaffoldin C CohesinRuminococcus flavefaciens
5D9P133948Glycoside Hydrolase 5Prevotella bryantii
5D9O133947Glycoside Hydrolase 5Prevotella bryantii
5D9N133946Glycoside Hydrolase 5Prevotella bryantii
5D9M133945Glycoside Hydrolase 5Prevotella bryantii
4YHG130962Glycoside Hydrolase 5Bacteroidetes bacterium AC2a
4YHE129494Glycoside Hydrolase 5Bacteroidetes bacterium AC2a
4W8B127728Glycoside Hydrolase 5Uncultured bacterium
4W8A127727Glycoside Hydrolase 5Uncultured bacterium
4W89127726Glycoside Hydrolase 5Uncultured bacterium
4W88127725Glycoside Hydrolase 5Uncultured bacterium
4W87127724Glycoside Hydrolase 5Uncultured bacterium
4W86127723Glycoside Hydrolase 5Uncultured bacterium
4W85127722Glycoside Hydrolase 5Uncultured bacterium
4W84127721Glycoside Hydrolase 5Uncultured bacterium
4KC8117245Glycoside Hydrolase 43Thermotoga petrophila RKU-1
4KC7117244Glycoside Hydrolase 43Thermotoga petrophila RKU-1
4N2O116090CohesinRuminococcus flavefaciens
4IU3109437CohesinRuminococcus flavefaciens
4IU2109436CohesinRuminococcus flavefaciens
4EYZ108431Cellulosomal proteinRuminococcus flavefaciens
4AEM106860Carbohydrate binding moduleEubacterium cellulosolvens
4AEK106648Carbohydrate binding moduleEubacterium cellulosolvens
4AFD106169Carbohydrate binding moduleEubacterium cellulosolvens
4BA6105916Carbohydrate binding moduleEubacterium cellulosolvens
4AFM105889Carbohydrate binding moduleEubacterium cellulosolvens
3VDH96587Glycoside Hydrolase 5Prevotella bryantii
2AGK39565His6 proteinSaccharomyces cerevisiae
5UHX156474CellulaseUncultured bacterium
2VG961544Glycoside Hydrolase 11Neocallimastix frontalis

The high-throughput gene expression studies (e.g. microarray, RNA-Seq) of microorganisms reveals highly significant molecular information on gene regulation. Analyzing the common gene expression patterns in microorganisms will play a crucial role in deciphering biological pathways and molecular mechanisms, dominant genes/proteins with greater importance. A best way to study the common gene expression pattern would be to perform a gene-expression metadata analysis. The publicly available gene expression datasets can be retrieved from the gene expression omnibus (GEO) and Array Express repositories by performing a simple search over all the database queries. Once retrieved these datasets can be analyzed using different methods and software packages summarized below (Table 7). Recent studies on metadata analysis of Phanerochaete chrysosporium and Postia placenta gene expression datasets have revealed the common differentially expressed gene patterns involved in lignocellulose metabolism. These studies have also reported the tentative molecular networks employed by these fungi during plant biomass degradation [76-79]. We have pictorially represented the tentative metadata analysis workflow implemented in previous studies, for analyzing and understanding the genomic and proteomic datasets (Figure 7).

 Table 6 

List of the publicly available resources in NCBI Chemicals databases that are related to the term “rumen”.

9 Conserved Biosystems: L-isoleucine biosynthesis V, Pyruvate fermentation to acetate VII, Oxalate degradation II, Phytol degradation, Coenzyme M biosynthesis I, Pyruvate fermentation to butanoate, L-isoleucine biosynthesis IV, Sulfur reduction I and Fatty acid alpha-oxidation II
4 Organism Specific Biosystems: Phytol degradation, Pyruvate fermentation to acetate VII, Isoleucine biosynthesis IV and Fatty acid alpha-oxidation
PubChem BioAssay
Insecticidal activity: Myzus persicae, Frankliniella occidentalis, Bemisia argentifolii, Plutella xylostella
Anti-feeding activity: Frankliniella occidentalis
Multicidal activity: Tetranychus urticae
Fungicidal activity: Botryotinia fuckeliana, Podosphaera fuligineam
2-In vitro rumen propionic acid test procedure
Antibacterial activity: Streptococcus equi 02I001, Streptococcus zooepidemicus 02H001, Staphylococcus aureus 01A005, Clostridium perfringens 10A002
 Figure 6 

Pictorial representation of metadata analysis workflow applied for analyzing and understanding the genomic metadata analysis approach;

J Genomics Image
 Table 7 

List of softwares (or) R-packages used for the metadata analysis of gene expression datasets. (Note: All the words written in bold are the names of the softwares (or) packages).

NameSoftware typeOperating systemProgramming language
MEMNAWeb user interfaceC++, JavaScript, Perl
Onto-Compare, PhenoGen, ExAtlas
NAWeb user interfaceNA
ImaGEO, ShinyMDE
NAWeb user interfaceR
BloaderPackage (or) ModuleUnix/Linux, Mac OS, WindowsGraphical user interface
MAAMDPackage (or) ModuleMac OS, WindowsNA
Package (or) Module
Unix/Linux, Mac OS, Windows, R
mixOmics, MetaOmics, CoGAPS, MetaQC, metaAnalyzeAll, metafor
metaMA, OrderedList, metahdep, YuGene, metaArray, RankAggreg,
MergeMaid, MAMA, NMF, GeneMeta, MetaDE, MetaSparseKmeans,
categoryCompare, IQRray
A-MADMANPackage (or) ModuleUnix/Linux, Mac OS, WindowsR, Python
MetaKTSPToolkit/SuiteUnix/Linux, Mac OS, WindowsR

2.5 Potential Applications

Rumen microbiota exhibit several potential applications in the dairy, feedstock, winery and brewery, pulp and paper, biofuel, biorefinery, textiles-detergent, food and pharmaceutical industries. Rumen microbiota can be potentially used in preparation of industrially important enzyme mixtures such as cellulases, xylanases, pectinases, amylases, lipases and proteases, which are widely used in various industrial processes [80, 81]. Recent studies have proved the importance of rumen microorganisms in the breakdown and conversion of lignocellulosic components (cellulose, hemicelluloses, pectin, lignin) to commercially important products such as bioethanol and other platform chemicals including hydrogen, butanol, iso-butanol, methane and other energy yielding products [82]. Enhancing the rate of digestion and metabolism of the feedstock by cattle is one of the major challenges in the dairy and feedstock industries. Several studies were continuously being conducted with rumen microorganisms and the enzymes secreted by these for pretreating the feedstock to enhance its digestion rate by ruminating animals [83]. Simultaneously, studies were also being conducted to develop genetically modified microorganisms (especially bacteria) to improve the process of digestion and enhance the rumen function [84]. Thus, understanding the microbial diversity of rumen will significantly benefit various industrial processes.

 Figure 7 

Pictorial representation of metadata analysis workflow applied for analyzing and understanding the proteomic metadata analysis approach.

J Genomics Image

3.0 Conclusions

All the above studies and reports strongly endorses that ruminating animals are solely dependent on their microbiota for their daily metabolism. Recent studies based on gut microbiomes of several ruminating animals, termite, mice and human have significantly enhanced the current knowledge about gut residing microorganisms. The JGI-MycoCosm and Hungate 1000 microbial genome sequencing projects revealed whole genome sequences of about 1087 fungal genomes and 501 rumen microorganisms. Although studies have reported that one milliliter of ruminal fluid contains approximately 105 to 106 protozoans and about 1010 to 1011 of bacterial inhabitants, there is still a long way in understanding the rumen microbiome and their role in physiology of ruminating animals. In this article we have extensively discussed the recent advancements in understanding the role of rumen microbiota in the degradation and metabolism of plant biomass. We have studied and listed all the genomic and proteomic studies based on rumen and ruminal microbiota. Further studies about the ruminal microbiota may benefit various industrial sectors significantly, including biofuel, biorefining, pretreatment of cattle feedstocks and several other processes. Our article can be used as a primer for understanding and focusing towards developing efficient recombinant microbial strains with higher plant biomass degrading abilities.


DOE: Department of Energy; JGI: Joint Genome Institute; GO: Gene Ontology; KOG: Eukaryotic orthologous groups; COG: Prokaryotic orthologous groups; CAZy: Carbohydrate active enzymes; SM: Secondary metabolites; KEGG: Kyoto encyclopedia of genes and genomes.


This work was supported by Natural Sciences and Engineering Research Council of Canada Funding (RGPIN-2017-05366) to Wensheng Qin and Mitacs Globalink Research Scholarship award to Ayyappa Kumar Sista Kameshwar.

Authors' contributions

AKSK has summarized the published data and drafted the manuscript. WQ and LPR contributed in revisiting and reviewing the manuscript. All authors read and approved the final manuscript.

Competing Interests

The authors have declared that no competing interest exists.


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Author contact

Corresponding address Corresponding author: luiz.ramosbr, wqinca

Received 2018-12-10
Accepted 2019-3-18
Published 2019-4-2

Citation styles

Kameshwar, A.K.S., Ramos, L.P., Qin, W. (2019). Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass. Journal of Genomics, 7, 31-45. https://doi.org/10.7150/jgen.32164.

Kameshwar, A.K.S.; Ramos, L.P.; Qin, W. Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass. J. Genomics 2019, 7, 31-45. DOI: 10.7150/jgen.32164.

Kameshwar AKS, Ramos LP, Qin W. Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass. J Genomics 2019; 7:31-45. doi:10.7150/jgen.32164. https://www.jgenomics.com/v07p0031.htm

Kameshwar AKS, Ramos LP, Qin W. 2019. Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass. J Genomics. 7:31-45.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
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