J Genomics 2016; 4:29-41. doi:10.7150/jgen.16885
De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome
1. Department of Biology, University of Nebraska at Omaha, Omaha, Nebraska 68182, USA;
2. Center for Environmental Health and Toxicology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA;
3. Department of Environmental, Agricultural and Occupational Health, University of Nebraska - Medical Center, Omaha, NE, 68198-6805, United States;
4. Departamento de Biología, Universidad de La Serena, La Serena, Chile;
5. Centro de Estudios Avanzados en Zonas Aridas, La Serena, Chile;
6. Instituto de Investigación Multidisciplinar en Ciencia y Tecnología, Universidad de La Serena, La Serena, Chile.
Schulze TT, Ali JM, Bartlett ML, McFarland MM, Clement EJ, Won HI, Sanford AG, Monzingo EB, Martens MC, Hemsley RM, Kumar S, Gouin N, Kolok AS, Davis PH. De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome. J Genomics 2016; 4:29-41. doi:10.7150/jgen.16885. Available from http://www.jgenomics.com/v04p0029.htm
Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina® platform and processed using PRINSEQ. The transcriptome assembly was performed using TRINITY assembler. Transcriptome validation was performed by functional characterization with KOG, KEGG, and GO analyses. Additionally, differential expression analysis highlights sex-specific expression patterns, and a list of endocrine and oxidative stress related transcripts are included.
Keywords: de novo transcriptome, assembly, catfish, Trichomycterus areolatus