Draft genome sequencing and assembly of Favolaschia claudopus CIRM-BRFM 2984 isolated from oak limbs

Favolaschia claudopus, a wood-inhabiting basidiomycete of the Mycenaceae family, is considered an invasive species that has recently spread from Oceania to Europe. The CIRM-BRFM 2984 strain of this fungus was originally isolated from a basidiome collected from the fallen limb of a decayed oak tree in Southwest France. The genome sequence of this strain shared characteristics with other Mycenaceae species, including a large genome size and enriched content of protein-coding genes. The genome sequence provided here will facilitate further investigation on the factors that contribute to the successful global dissemination of F. claudopus.


Introduction
The genus Favolaschia belongs to the Mycenaceae family within the order Agaricales.It is known for its poroid basidiomes that are found in dead plant materials worldwide.Previously considered as a variety of Favolaschia calocera, F. claudopus (Singer) Q.Y.Zhang & C. Dai has recently been elevated to the species status (1).F. claudopus is a wood-inhabiting fungus classified as an invasive species that has recently spread from Oceania to Europe (2; 3; 1).It is suspected that the introduction of this species may be favored by the presence of black locust (Robinia pseudoacacia), which is the primary host of F. claudopus.The adaptive capabilities of this species have raised concerns among mycologists as they could potentially endanger local fungal species (4; 5).The first specimens of F. claudopus in metropolitan France were observed in Pyrénées-Atlantiques

Ivyspring
International Publisher (southwest France).We have carried out genome sequencing of the F. claudopus strain CIRM-BRFM 2984 as part of the 1000 Fungal Genomes (1KFG) project, an extensive international research initiative focused on sequencing the genomes of various fungal taxa, in collaboration with the Joint Genome Institute of the U.S. Department of Energy (6).
CIRM-BRFM 2984, a strain of F. claudopus, was isolated from a basidiome collected from the fallen branch of a decayed Quercus tree in Agnos, France, in November 2018 (refer to Fig. 1 for visual representation).The basidiome was dried and preserved using voucher number AMB49273.The isolation process involved transferring tissues from the basidiome to agar plates containing malt extract supplemented with antibiotics (0.025% chloramphenicol and 0.04% gentamicin).
Successive subcultures without antibiotics were performed to verify the absence of contaminants.To confirm the identity of the isolated strain, the rDNA barcoding region ITS1-5,8S-ITS2 was PCR-amplified and sequenced, as previously described (7).Following molecular authentication, the strain was deposited in the Biological Resource Center CIRM-CF (International Center of Microbial Resources, Marseille, France; https://doi.org/10.15454/KJQW-SJ57; www.cirm-fungi.fr)under the accession number CIRM-BRFM 2984.
Further research, including comparative genomic analysis with other Mycena species, will aid in determining whether the expansive genome of F. claudopus has facilitated its ability to disperse and establish itself in diverse ecological niches.