J Genomics 2018; 6:122-126. doi:10.7150/jgen.27741

Research Paper

Genomic Analysis of Rhodococcus sp. Br-6, a Bromate Reducing Bacterium Isolated From Soil in Chiba, Japan

Kohei Ito1,2, Masafumi Harada1,2, Nobuyoshi Nakajima3, Shigeki Yamamura3,4, Masaru Tomita1,2, Haruo Suzuki1,2✉, Seigo Amachi5

1. Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan
2. Faculty of Environment and Information Studies, Keio University, Fujisawa, Kanagawa, Japan
3. Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan
4. Center for Regional Environmental Research, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan
5. Graduate School of Horticulture, Chiba University, Matsudo City, Chiba, Japan

Abstract

Bromate is a byproduct of the ozone disinfection of drinking water. It is a genotoxic carcinogen and causes renal cell tumors in rats. Physicochemical removal of bromate is very difficult, making microbial reduction of bromate to bromide a promising approach to eliminate bromate from water. Rhodococcus sp. Br-6, isolated from soil, can efficiently reduce bromate by using acetate as an electron donor. We determined the draft genome sequence of Rhodococcus sp. Br-6 for the potential practical application of the bromate-reducing bacterium. Core genome phylogeny suggests that the Br-6 strain is most closely related to R. equi. The Br-6 genome contains genes encoding multiple isoforms of diaphorase, previously found to be involved in Br-6-mediated bromate reduction. The genes identified in the present study could be effective targets for experimental studies of microbial bromate reduction in the future.

Keywords: Bromate reducing bacterium, Rhodococcus sp. Br-6, Genome, Phylogeny, Diaphorase.

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How to cite this article:
Ito K, Harada M, Nakajima N, Yamamura S, Tomita M, Suzuki H, Amachi S. Genomic Analysis of Rhodococcus sp. Br-6, a Bromate Reducing Bacterium Isolated From Soil in Chiba, Japan. J Genomics 2018; 6:122-126. doi:10.7150/jgen.27741. Available from http://www.jgenomics.com/v06p0122.htm