J Genomics 2017; 5:91-98. doi:10.7150/jgen.21588

Research Paper

Genome Comparison of Pseudomonas fluorescens UM270 with Related Fluorescent Strains Unveils Genes Involved in Rhizosphere Competence and Colonization

Julie E. Hernández-Salmerón1, Gabriel Moreno-Hagelsieb2, Gustavo Santoyo1✉

1. Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México;
2. Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada.

Abstract

Pseudomonas fluorescens UM270 is a rhizosphere-colonizing bacterium that produces multiple diffusible and volatile compounds involved in plant growth-promoting activities. Strain UM270 exhibits excellent biocontrol capacities against diverse fungal pathogens. In a previous study, the general UM270 genome characteristics were published. Here, we report a deeper analysis of its gene content and compare it to other P. fluorescens strains to unveil the genetic elements that might explain UM270's great colonizing and plant growth-promoting capabilities. Our analyses found high variation in genome size and gene content among the eight Pseudomonas genomes analyzed (strains UM270, Pf0-1, A506, F113, SBW25, PICF-7, UK4 and UW4). A core genome of 3,039 coding DNA sequences (CDSs) was determined, with 599 CDSs present only in the UM270 genome. From these unique UM270 genes, a set of 192 CDSs was found to be involved in signaling, rhizosphere colonization and competence, highlighted as important traits to achieve an effective biocontrol and plant growth promotion.

Keywords: Pseudomonas, Comparative Genomics, Rhizosphere, Biocontrol, Plant Growth-Promotion Bacteria.

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How to cite this article:
Hernández-Salmerón JE, Moreno-Hagelsieb G, Santoyo G. Genome Comparison of Pseudomonas fluorescens UM270 with Related Fluorescent Strains Unveils Genes Involved in Rhizosphere Competence and Colonization. J Genomics 2017; 5:91-98. doi:10.7150/jgen.21588. Available from http://www.jgenomics.com/v05p0091.htm