J Genomics 2012; 1:22-28. doi:10.7150/jgen.3781

Review

Adipogenic Transcriptome Profiling Using High Throughput Technologies

Urmila Basu, Josue Moura Romao, Le Luo Guan^✉

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada

Abstract

The recent technological innovations in the area of functional genomics, gene expression/transcriptomic profiling can provide new insights to understand the molecular basis of adipogenesis. The focus of this review is to highlight the recent advances in our understanding of the complex interplay of gene expression events and the regulatory mechanisms of adipogenesis in mouse cell lines, humans and farm animals. All these studies have employed the availability of constantly evolving high throughput 'omics' technologies including microarrays, RNA-Seq, chromatin immunoprecipitation, next generation sequencing, RNAi, miRNA profiling and quantitative PCR arrays.

Keywords: transcriptome, adipogenesis, functional genomics, gene expression